Collagen III alpha 1/COL3A1 Rabbit Polyclonal Antibody
| Cat Number: | AB-82620 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1201-1301 of human Collagen III alpha 1/COL3A1. |
| Reactivity: | Hu , Ms |
| Applications: | Western Blot: 1:2000 – 1:10000 |
| Molecular Weight: | 150kDa |
| Purification: | Affinity purification |
| Synonyms: | EDS4A; EDSVASC; PMGEDSV; Collagen III alpha 1/COL3A1 |
| Background: | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of lysates from HeLa cells using Collagen III alpha 1/COL3A1 Rabbit pAb at 1:10000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL West Pico Plus.
Exposuretime:90s.
Immunofluorescence analysis of RD cells using Collagen III alpha 1/COL3A1 Rabbit pAb at dilution of 1:100.
Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 Rabbit pAb at dilution of 1:100.
Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
Blue: DAPI for nuclear staining.