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COLLAGEN V / COL5A1 Rabbit Polyclonal Antibody

Cat Number: AB-84803
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human Collagen V alpha1. AA range:301-350
Reactivity: Human,Mouse,Rat
Applications:

Western Blot: 1/500 – 1/2000.
Immunohistochemistry: 1/100 – 1/300
Immunofluorescence: 1/200 – 1/1000
Molecular Weight: 200kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: COL5A1; Collagen alpha-1(V) chain
Background:

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI
collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the
heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants.
Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-84803-1

Western Blot analysis of various cells using COL5A1 Polyclonal Antibody.

AB-84803-2

Western blot analysis of lysates from HeLa, and HUVEC, and HepG2 cells, using Collagen V alpha1 Antibody. The
lane on the right is blocked with the synthesized peptide.

AB-84803-3

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen V alpha1 Antibody.
The picture on the right is blocked with the synthesized peptide.

AB-84803-4

Immunofluorescence analysis of HeLa cells, using Collagen V alpha1 Antibody. The picture on the right is blocked
with the synthesized peptide.