COX 2
| Cat Number: | MAB-94444 |
|---|---|
| Size: | 100 ug |
| Clone: | D5H5 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB 1:1000-1:2000, IHC-P 1:100, IF-IP 1:100, Unmasking buffer: Citrate |
| Molecular Weight: | 74 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His108 of human Cox2 protein. |
| Background: | Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer’s disease by potentiating γ-secretase activity and β-amyloid generation (6).Cox2 (D5H5) XP® Rabbit mAb recognizes endogenous levels of total Cox2 protein. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50%glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles |
tfistern blot analysis of extracts of various cell
lines, using Cox2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG
(H+L) at 1:10000 dilution. Lysates/proteins:
25ug per lane. Blocking buffer: 3% nonfat dry milk
in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.
Immunohistochemistry of paraffinembedded
human liver cancer using Cox2
antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells
using Cox2 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.
Application Immunohistochemistry(IHC)
Sample RAW264.7,Mouse kidney
Dilution 1:200
Incubatition time 12h
Temperature 4 ℃
Exposure unknow
Observed Band unknow kDa
Remarks Immunohistochemistry of paraffin-embedded mouse kidney tissue using ptgs2(cox-2 )antibody at dilution of 1:100 (x100 lens).
Application Western blot(WB)
Sample RAW264.7,Mouse kidney
Loading Amount 15 ug
Dilution 1:1000
Incubatition time over night
Temperature 4 ℃
Exposure 30s
Observed Band unknow kDa
Remarks Western blot analysis of extracts of mouse RAW264.7 cells, using ptgs2 antibody 1:1000 .
Application Western blot (WB) Sample
Mouse embryonal fibroblast cell
Loading Amount 15 ug
Dilution 1:1000
Incubatition time over night
Temperature 4 ℃
Exposure 15s
Remarks Western blot analysis of extracts of Mouse embryonal fibroblast cell, using antibody at 1:1000 dilution.
References
References for Cox2 Polyclonal Antibody Product:
Cox2 Polyclonal Antibody
Journal:Journal of ethnopharmacology
Application:IHC
IF:2.980
Species:unknown
PMID:28238827
Title:Rhizoma Smilacis Glabrae inhibits pathogen-induced upper genital tract inflammation in rats through suppression of NF-κB pathway
References for Cox2 Polyclonal Antibody
Product: Cox2 Polyclonal Antibody
Journal: Vascular Pharmacology
Application: WB
IF: 3.715
Species: Rat
PMID: 28797762
Title: Ceramide enhances COX-2 expression and VSMC contractile hyperreactivity via ER stress signal activation
References for Cox2 Polyclonal Antibody
Product: Cox2 Polyclonal Antibody
Journal: Molecular neurobiology
Application: WB
IF: 6.189
Species: Mouse
PMID: 27957686
Title:Curcumin Attenuates Colistin-Induced Neurotoxicity in N2a Cells via Anti-inflammatory Activity, Suppression of Oxidative Stress, and Apoptosis
References for Cox2 Polyclonal Antibody
Product:Cox2 Polyclonal Antibody
Journal:Journal of experimental & clinical cancer research : CR
Application:
WB, IHC
IF:6.21
Species:Mouse
PMID:30119635
Title:IL-33 facilitates proliferation of colorectal cancer dependent on COX2/PGE 2
For Research use only
IMMUNOLOGICAL SCIENCES
Web-site: www.immunologicalsciences.com – E-Mail: info@immunologicalsciences.com
References for Cox2 Polyclonal Antibody
Product:Cox2 Polyclonal Antibody
Journal:Oxidative Medicine and Cellular Longevity
Application:WB
IF:.93
Species:Mouse
PMID:30159118
Title: Anti-Inflammatory Effect of a Polyphenol-Enriched Fraction from Acalypha wilkesiana on Lipopolysaccharide-Stimulated RAW 264.7 Macrophages and Acetaminophen-Induced Liver Injury in Mice.