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COX4

Cat Number: MAB- 94626
Conjugate: Unconjugated
Size: 100 ug
Clone: 6C8
Concentration: 1mg/ml
Host: Ms
Isotype: IgG1
Immunogen: A synthetic peptide of human COX IV
Reactivity: Hu, Ms, Rt, Hm, Mk, Gt
Applications:

Western Blot: 1:1000 – 1:4000
Immunohistochemistry: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200
Immunoprecipitation: 1:20 – 1:50
Flow cytometry: 1:20 – 1:50

Molecular: 17kDa
Purification: Aff. Pur
Synonyms: COX4I1;COX IV-1;COX4;COX4-1;COXIV;COXIV-1;COX IV
Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-94626-1

Western blot analysis of extracts of various cell lines, using COX IV antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. Exposure time: 30s.

MAB-94626-2

Immunofluorescence analysis of Hela Cell.1, AF-10 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight).
COX IV monoclonal antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 (was diluted at 1:1000 (room temperature, 50 min.).
Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min.).

References

References for COX IV Mouse mAb
Product:COX IV Mouse mAb
Journal:Plos One
Application:WB
IF:2.804
Species:Homo sapiens
PMID:25933104
Title:Mechanism of Arctigenin-Induced Specific Cytotoxicity against Human Hepatocellular Carcinoma Cell Lines: Hep G2 and SMMC7721

References for COX IV Mouse mAb
Product:COX IV Mouse mAb
Journal:Nature Communications
Application:WB
IF:11.87
Species:Danio rerio
PMID:32161263
Title:Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly

References for COX IV Mouse mAb
Product:COX IV Mouse mAb
Journal:Scientific Reports
Application:WB
IF: 4.01
Species:Danio rerio
PMID:31827208
Title:Fundc1 is necessary for proper body axis formation during embryogenesis in zebrafish

References for COX IV Mouse mAb
Product:COX IV Mouse mAb
Journal:Redox Biol
Application:WB
IF:9.98
Species:Homo sapiens
PMID:32829253
Title:HIF-1α-BNIP3-mediated mitophagy in tubular cells protects against renal ischemia/reperfusion injury

References for COX IV Mouse mAb
Product:COX IV Mouse mAb
Journal:Acta Pharmacol Sin
Application:WB
IF:5.06
Species:Homo sapiens
PMID:32747719
Title:LW-213 induces cell apoptosis in human cutaneous T-cell lymphomas by activating PERK–eIF2α–ATF4–CHOP axis