CREB-1 (phospho Ser133) Rabbit Polyclonal Antibodty
| Cat Number: | ABP-0333 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133. AA range:100-149 |
| Reactivity: | Human;Mouse;Rat;Pig |
| Applications: | Western Blot: 1/500 – 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 – 1/300 Immunoprecipitation: 2-5 ug/mg lysate. |
| Molecular Weight: | 37kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1 |
| Background: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid repeated freeze-thaw cycles. |
Western blot analysis of lysates from Jurkat and 293 cells treated with UV,using CREB (Phospho-Ser133) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,CREB-1 (phospho Ser133) Polyclonal Antibody was diluted at 1:200(4°C,overnight).
2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative
control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell.1,CREB-1 (phospho Ser133) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488
Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).