CREB 2 / ATF4
| Cat Number: | AB-82156 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 |
| Reactivity: | Hu |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 50kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ATF4;CREB-2;CREB2;TAXREB67;TXREB |
| Background: | This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of HeLa cells, using ATF4 antibody at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.
Immunofluorescence analysis of HeLa cells using ATF4 antibody at dilution of
1:100. Blue: DAPI for nuclear staining.
References
References for ATF4 Rabbit pAb
Product:ATF4 Rabbit pAb
Journal:Biochimica et biophysica acta
Application:WB
IF:
Species:Mus musculus
PMID: 30017912
Title: Selenoprotein S silencing triggers mouse hepatoma cells apoptosis and necrosis involving in intracellular calcium imbalance and ROS-mPTP-ATP.
References for ATF4 Rabbit pAb
Product: ATF4 Rabbit pAb
Journal: Cellular and molecular neurobiology
Application: WB
IF: 3.89
Species: Rattus norvegicus
PMID: 30607811
Title: MALAT1 Up-Regulator Polydatin Protects Brain Microvascular Integrity and Ameliorates Stroke Through C/EBPβ/MALAT1/CREB/PGC-1α/PPARγ Pathway.
References for ATF4 Rabbit pAb
Product: ATF4 Rabbit pAb
Journal: ACS Chemical Neuroscience
Application: WB
IF: 3.86
Species: Rattus norvegicus
PMID: 32186847
Title: Astrocyte-Derived Lipocalin-2 Is Involved in Mitochondrion-Related Neuronal Apoptosis Induced by Methamphetamine
References for ATF4 Rabbit pAb
Product: ATF4 Rabbit pAb
Journal: Oxidative Medicine and Cellular Longevity
Application: WB
IF: 4.86
Species: Homo sapiens
PMID: 31687081
Title: Bovine Herpesvirus 1 Productive Infection Led to Inactivation of Nrf2 Signaling through Diverse