CXCL10 Rabbit Polyclonal Antibody
| Cat Number: | AB-80549 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-98 of human CXCL10/IP-10 |
| Reactivity: | Human, Mouse |
| Applications: | Western Blot: 1:1000 – 1:5000 |
| Molecular Weight: | 11kDa |
| Purification: | Affinity purification |
| Synonyms: | C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10; CXCL10/IP-10 |
| Background: | This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. This gene may also be a key regulator of the ‘cytokine storm’ immune response to SARS-CoV-2 infection. |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of THP-1, using CXCL10/IP-10 antibody at 1:3000 dilution.THP-1 cells
were treated by LPS for 6 hours,Brefeldin A for 3 hours and IFNγ (50ng/ml) for 8 hours of stimulation.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.Exposure time: 30s.
Immunofluorescence analysis of NIH/3T3 cells using CXCL10/IP-10 Rabbit pAb at dilution of 1:200 (40x lens).
Blue: DAPI for nuclear staining.