CXCL10 Rabbit Polyclonal Antibody

Cat Number: AB-80549
Conjugate: Unconjugated
Size: 100μg
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-98 of human CXCL10/IP-10
Reactivity: Human, Mouse

Western Blot: 1:1000 – 1:5000
Immunofluorescence: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200

Molecular Weight: 11kDa
Purification: Affinity purification
Synonyms: C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10; CXCL10/IP-10

This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. This gene may also be a key regulator of the ‘cytokine storm’ immune response to SARS-CoV-2 infection.

Form: Liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of THP-1, using CXCL10/IP-10 antibody at 1:3000 dilution.THP-1 cells
were treated by LPS for 6 hours,Brefeldin A for 3 hours and IFNγ (50ng/ml) for 8 hours of stimulation.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.Exposure time: 30s.


Immunofluorescence analysis of NIH/3T3 cells using CXCL10/IP-10 Rabbit pAb at dilution of 1:200 (40x lens).
Blue: DAPI for nuclear staining.