|Immunogen:||Recombinant protein of human CXCR2.|
|Reactivity:||Hu, Ms, Rt|
Western Blot: 1:500 – 1:2000
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively, spliced variants, encoding the same protein, have been identified.
|Buffer:||PBS with 0.02% sodium azide,50% glycerol,pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of
various cell lines, using CXCR2
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
Detection: ECL West Pico Plus
Exposure time: 5s.
Immunohistochemistry of paraffin
Embedded Mouse spleen using
CXCR2 Rabbit pAb ) at dilution
of 1:100 (40x lens).
Immunofluorescence analysis of Jurkat cells using CXCR2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.