Cyclophilin B/PPIB
| Cat Number: | AB-83838 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 34-216 of human PPIB |
| Reactivity: | Hu, Rt, Ms |
| Applications: | Western Blot: 1:500-1:2000 |
| Molecular Weight: | 24kDa |
| Purification: | Aff. Pur. |
| Synonyms: | PPIB; CYP-S1; CYPB; HEL-S-39; OI9; SCYLP; peptidylprolyl isomerase B |
| Background: | he protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and Blymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this this protein that give rise to recessive forms of osteogenesis imperfecta. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using PPIB antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.