|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 34-216 of human PPIB|
|Reactivity:||Hu, Rt, Ms|
Western Blot: 1:500-1:2000
|Synonyms:||PPIB; CYP-S1; CYPB; HEL-S-39; OI9; SCYLP; peptidylprolyl isomerase B|
he protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and Blymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this this protein that give rise to recessive forms of osteogenesis imperfecta.
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of various cell lines, using PPIB antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.