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DAPK2

Cat Number: AB-83998
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:200 -1:1000
Immunohistochemistry: 1:20 -1:100

Molecular: 12kDa
Purification: Aff. Pur.
Synonyms: COX6A1; CMTRID; COX6A; COX6AL; cytochrome c oxidase subunit 6A1
Background:

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83998-1.jpg
AB-83998-1.jpg

Western blot analysis of extracts of various cell lines, using COX6A1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human liver cancer using COX6A1 antibody at dilution of 1:100 (40x lens).