DDDDK-Tag (binds to flag sequence) Rabbit Polyclonal Antibody
| Cat Number: | AB-J6547 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | DDDDK synthetic peptide conjugated to KLH. |
| Reactivity: | Species independent |
| Applications: | Western Blot: 1/1000 – 1/3000 |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag |
| Background: | The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. |
| Form: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000