DDDDK-Tag Rabbit Polyclonal Antibody

Cat Number: AB-84802
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: DDDDK synthetic peptide conjugated to KLH.
Reactivity: Species independent

Western Blot: 1/1000 – 1/3000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein
localization and so on.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector
overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000