Desmin
Cat Number: | AB-82627 |
---|---|
Conjugate: | Unù |
Size: | 100 ug |
Clone: | POLY |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | IgG |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-470 of human Desmin. |
Reactivity: | Hu,Ms,Rt |
Applications: | Western Blot: 1:500 – 1:2000 |
Molecular Weight: | 55kDa |
Purification: | Aff. Pur. |
Synonyms: | DES;CSM1;CSM2;LGMD2R;desmin;Desmin |
Background: | This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. |
Form: | Liquid |
Buffer: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
![AB-82627-1](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-82627-1.jpg)
Western blot analysis of extracts of C2C12 cells, using Desmin antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECLWest Pico Plus. Exposure time: 30s
![AB-82627-2](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-82627-2.jpg)
Immunohistochemistry of paraffin embedded
human colon using Desmin
Rabbit pAb at dilution of 1:100
(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
before commencing with IHC staining protocol
![AB-82627-3](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-82627-3.jpg)
Immunohistochemistry of paraffin embedded mouse lung using Desmin Rabbit pAb at dilution of 1:100 (40x lens).
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
before commencing with IHC staining protocol.
![AB-82627-4](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-82627-4.jpg)
Immunofluorescence analysis of rat heart using Desmin Rabbit pAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
References:
Product:Desmin Rabbit pAb
Journal:Journal Of Cellular Physiology
Application:IHC
IF:6.384
Species:Mus musculus
PMID:33230845
Title:SOD3 deficiency induces liver fibrosis by promoting hepatic stellate cell activation and epithelial–mesenchymal transition
References:
Product:Desmin Rabbit pAb
Journal:Plant Physiol
Application:WB
IF:8.34
Species:Triticum aestivum L.
PMID:33576803
Title:A semi-dominant NLR allele causes whole-seedling necrosis in wheat