Dio-1 Rabbit Polyclonal Antibody

Cat Number: AB-84782
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human DIDO1. AA range:161-210
Reactivity: Human, Mouse

Western Blot: 1/500 – 1/2000
Immunohistochemistry: 1/100 – 1/300
ELISA: 1/10000

Molecular Weight: 244 kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: DIDO1; C20orf158; DATF1; KIAA0333; Death-inducer obliterator 1; DIO-1; hDido1; Death-associated transcription factor 1; DATF-1

Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.

Western Blot analysis of various cells using Dio-1 Polyclonal Antibody diluted at 1:2000


Western blot analysis of lysates from 293 cells, using DIO1 Antibody. The lane on the right is blocked with the synthesized peptide.


Immunohistochemical analysis of paraffin-embedded human tonsil.
1, Antibody was diluted at 1:200(4° overnight).
2,Tris-EDTA,pH9.0 was used for antigen retrieval.
3,Secondary antibody was diluted at 1:200(room temperature, 45min).