Dopamine Decarboxylase (DDC)
| Cat Number: | AB-83411 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human DDC |
| Reactivity: | Rt, Ms, Hu, |
| Applications: | WB 1:1000 |
| Molecular Weight: | 54kDa |
| Purification: | Aff. Pur. |
| Background: | The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Mµltiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles |
Western blot analysis of extracts of various
cell lines, using DDC antibody at
1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit
IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 60s.