Doublecortin Rabbit Polyclonal Antibody

Cat Number: AB-84801
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 265-365 of human Doublecortin
Reactivity: Human,Mouse, Rat

Western Blot: 1:500 – 1:1000
Immunofluorescence: 1:50 – 1:200
Immunocytoichemistry: 1:50 – 1:200

Molecular Weight: 41kDa
Purification: Affinity purification
Synonyms: DC; DBCN; LISX; SCLH; XLIS; Doublecortin

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia (“double cortex” syndrome) in females and lissencephaly (“smooth brain” syndrome) in males. Multiple transcript variants encoding different isoforms
have been found for this gene.

Form: Liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300,50% glycerol

Western blot analysis of extracts of mouse brain, using Doublecortin antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.


Immunofluorescence analysis of SH-5Y5Y cells using Doublecortin Rabbit pAb at dilution of 1:20 (40x lens). Blue: DAPI for
nuclear staining.