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DRP1

Cat Number: AB-83896
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 411-710 of human DRP1.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 -1:1000
Immunohistochemistry: 1:50 -1:100
Immunofluorescence: 1:50 -1:100

Molecular: 82kDa
Purification: Aff. Pur.
Synonyms: DNM1L; DLP1; DRP1; DVLP; DYMPLE; EMPF; EMPF1; HDYNIV; dynamin 1 like
Background:

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer’s disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83896-1.jpg

Western blot analysis of extracts of various cell lines, using DRP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.

AB-83896-2.jpg

Western blot analysis of extracts of rat thymus, using DRP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

AB-83896-3.jpg

Immunohistochemistry of paraffin-embedded human liver injury using DRP1 antibody at dilution of 1:100 (40x lens).

AB-83896-4.jpg

Immunofluorescence analysis of HeLa cells using DRP1 antibody. Blue: DAPI for nuclear staining