DYKDDDK
| Cat Number: | MAB-10418 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | F-tag-01 |
| Concentration: | 1mg/mL |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide: DYKDDDDK conjugated to KLH |
| Reactivity: | All Species |
| Applications: | Immunocytochemistry |
| Purification: | Purified by protein-A affinity chromatography |
| Background: | The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function. |
| Form: | Liquid |
| Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage: | Store at 2-8oC. Do not freeze |
Fig. 1A Fusion nuclear protein
Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.
1A – fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)
Fig. 1B Cell nuclei
1B – cell nuclei stained with DAPI (blue)
1C – merged figures – confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)
References
*Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt
signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J.
2006 Jun 7;25(11):2326-37.
*Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M,
Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription
driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of
print]