Cat Number: MAB-10418
Conjugate: Unconjugated
Size: 100 ug
Clone: F-tag-01
Concentration: 1mg/mL
Host: Ms
Isotype: IgG1
Immunogen: Synthetic peptide: DYKDDDDK conjugated to KLH
Reactivity: All Species

Western Blotting

Purification: Purified by protein-A affinity chromatography

The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Form: Liquid
Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage: Store at 2-8oC. Do not freeze

Fig. 1A Fusion nuclear protein
Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.
1A – fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)


Fig. 1B Cell nuclei
1B – cell nuclei stained with DAPI (blue)


1C – merged figures – confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)


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Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription
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