|Immunogen:||Synthetic peptide: DYKDDDDK conjugated to KLH|
|Purification:||Purified by protein-A affinity chromatography|
The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
|Buffer:||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage:||Store at 2-8oC. Do not freeze|
Fig. 1A Fusion nuclear protein
Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.
1A – fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)
Fig. 1B Cell nuclei
1B – cell nuclei stained with DAPI (blue)
1C – merged figures – confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)
*Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt
signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J.
2006 Jun 7;25(11):2326-37.
*Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M,
Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription
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