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E-cadherin Rabbit Polyclonal Antibody

Cat Number: ABN10278
Conjugate: Unconjugated
Size: 100 ug
Concentration: 1 mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human Cadherin. range: 1-50
Reactivity: Human,Mouse,Rat
Applications: WB 1:500-1:1000; IHC 1:100-1:200; ICC/IF 1:100-1:200; ELISA 1:10000-1:20000
Molecular: Calculated MW: 97 kDa
Purification: Affinity purification
Synonyms: CDH1; CDHE; UVO; Cadherin-1; CAM120/80; Epithelial cadherin; E-cadherin
Background:

Anti-E-cadherin Antibody detects endogenous levels of total E-cadherin protein.
CDH1 (cadherin 1) codes for a transmembrane glycoprotein E-cadherin that is located on human chromosome 16q22.1. It is a tumor suppressor gene, which is also known as Cdh1p/Hct1p, Fizzy-related, Ste9, or Srw1.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% New type preservative N.
Storage: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using E-Cadherin Antibody. The picture on the right is Blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using E-Cadherin Antibody. The picture on the right is Blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, using Cadherin Antibody.The lane of the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, using Cadherin Antibody.The lane of the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-lung tissue. 1,E-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: mergeofA+B

Immunofluorescence analysis of rat-lung tissue. 1,E-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: mergeofA+B