Top

EIF2A Rabbit Monoclonal Antibody

Cat Number: MAB-94711
Conjugate: Unconjugated
Size: 100 ug
Clone: D7D3
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A recombinant protein fragment corresponding to a sequence of human EIF2A
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:1000;IF: 1:50; ICC 1:50
Molecular Weight: 38kDa
Purification: Aff. Pur.
Synonyms: EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1
Background:

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis
initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding
occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3
nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta
subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161).
The rate of formation of the ternary complex is modulated by the phosphorylation state
of EIF2-alpha
Form: Liquid
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
MAB-94711-1.jpg

Western blot analysis of extracts of various cell lines, using eIF2α antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

MAB-94711-2.jpg

Western blot analysis of extracts of various cell lines, using eIF2α antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

MAB-94711-3.jpg

Immunofluorescence analysis of HeLa using eIF2α Rabbit MAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.