Factor VIII Rabbit Polyclonal Antibody
| Cat Number: | ABN00399 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μL |
| Clone: | Polyclonal |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Factor VIII. AA range:2161-2210 |
| Reactivity: | Human,Mouse |
| Applications: | WB 1:500-1:1000,IHC 1:50-1:100,ELISA 1:5000-1:20000 |
| Molecular: | Calculated MW: 267 kDa; Observed MW: 300 kDa |
| Purification: | Affinity Purification |
| Synonyms: | F8; F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component |
| Background: | F8: coagulation factor VIII, procoagulant component. This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles. |