FGFR4 Rabbit Monoclonal Antibody
| Cat Number: | MAB-94770 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC1474 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700-802 of human FGFR4. |
| Reactivity: | Human, Mouse |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 95KDa/125KDa |
| Purification: | Affinity purification |
| Synonyms: | TKF; JTK2; CD334 |
| Background: | The protein encoded by this gene is a tyrosine kinase and cell surface receptor for fibroblast growth factors. The encoded protein is involved in the regulation of several pathways, including cell proliferation, cell differentiation, cell migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. This protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion interacts |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using FGFR4 Rabbit mAb at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.
Western blot analysis of extracts of Mouse lung, using FGFR4 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.
Immunohistochemistry of paraffinembedded human placenta using FGFR4 Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Referemces:
References for FGFR4 Rabbit mAb
Product:FGFR4 Rabbit mAb
Journal:The Journal of nutritional biochemistry
Application:WB
IF:6.11
Species:Rattus norvegicus
PMID:35613674
Title:Antarctic Krill Oil ameliorates liver injury in rats exposed to alcohol by regulating bile acids metabolism and gut microbiota
References for FGFR4 Rabbit mAb
Product:FGFR4 Rabbit mAb
Journal:Frontiers in bioengineering and biotechnology
Application:WB
IF:6.06
Species:Homo sapiens
PMID:36532586
Title: Generation of multicellular tumor spheroids with micro-well array for anticancer drug combination screening based on a valuable biomarker of hepatocellular carcinoma – PMC