Fibronectin Rabbit Polyclonal Antibody
| Cat Number: | AB-84281 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids2200-2355 of human Fibronectin. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 272KDa |
| Purification: | Aff. Pur. |
| Synonyms: | FN1;CIG;ED-B;FINC;FN;FNZ;GFND;GFND2;LETS;MSF;fibronectin |
| Background: | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various lysates, using Fibronectin antibody at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunohistochemistry of paraffinembedded human lung cancer using Fibronectin antibody at dilution
of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining
protocol.
Immunohistochemistry of paraffinembedded mouse testis leydig using Fibronectin antibody (A16678) at dilution
of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining
protocol.
Immunofluorescence analysis of HepG2 cells using Fibronectin Rabbit pAb at dilution of 1:50 (40x lens).
Blue: DAPI for nuclear staining.
References
References for Fibronectin Rabbit pAb
Product: Fibronectin Rabbit pAb
Journal: Biomedicine & Pharmacotherapy
Application: WB
IF: 3.74
Species: Homo sapiens,Mus musculus
PMID: 31810140
Title: LncRNA GAS5 exacerbates renal tubular epithelial fibrosis by acting as a competing endogenous RNA of miR-96-5p
References for Fibronectin Rabbit pAb
Product: Fibronectin Rabbit pAb
Journal:biomed pharmacother
Application: WB
IF: 4.54
Species: Homo sapiens
PMID: 32768952
Title: Luteolin suppresses epithelial-mesenchymal transition and migration of triple-negative breast cancer cells by inhibiting YAP/TAZ activity
References for Fibronectin Rabbit pAb
Fibronectin Rabbit pAb
Journal: Front Pharmacol
Application: IHC
IF: 4.22
Species: Mus musculus
PMID: 32848753
Title: Trimetazidine Inhibits Renal Tubular Epithelial Cells to Mesenchymal Transition in Diabetic Rats via Upregulation of Sirt1
References for Fibronectin Rabbit pAb
Product:Fibronectin Rabbit pAb
Journal:Biochem Biophys Res Commun
Application: WB
IF: 2.98
Species: Homo sapiens
PMID: 32859376
Title: GOLPH3 silencing inhibits adhesion of glioma U251 cells by regulating ITGB1 degradation under serum starvation
References for Fibronectin Rabbit pAb
Product: Fibronectin Rabbit pAb
Journal: Life Sci
Application: WB
IF: 3.64
Species: Homo sapiens,Mus musculus
PMID: 32918978
Title: Oroxylin A reversed Fibronectin-induced glioma insensitivity to Temozolomide by suppressing IP3R1/AKT/尾-catenin pathway
References for Fibronectin Rabbit pAb
Product:Fibronectin Rabbit pAb
Journal: Pharmacol Res
Application: WB
IF: 5.89
Species: Mus musculus
PMID: 32492489
Title: Oroxylin A suppresses ACTN1 expression to inactivate cancer-associated fibroblasts and restrain breast cancer metastasis
References for Fibronectin Rabbit pAb
Product: Fibronectin Rabbit pAb
Journal: Evid Based Complement Alternat Med
Application:WB, IHC
IF:1.84
Species:Mus musculus
PMID:33688363
Title: Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation
References for Fibronectin Rabbit pAb
Product:Fibronectin Rabbit pAb
Journal:Phytomedicine
Application:WB
IF:5.34
Species:Homo sapiens,Mus musculus
PMID:30785018
Title:Resveratrol exerts dose-dependent anti-fibrotic or pro-fibrotic effects in kidneys: A potential risk to individuals with impaired kidney function.
References for Fibronectin Rabbit pAb
Product: Fibronectin Rabbit pAb
Journal: Stem cell research & therapy
Application: WB
IF: 6.832
Species: Mus musculus
PMID: 30704535
Title: Microvesicles from human adipose stem cells promote wound healing by optimizing cellular functions via AKT and ERK signaling pathways.
References for Fibronectin Rabbit pAb
Product:Fibronectin Rabbit pAb
Journal:Molecular cancer
Application:WB
IF:27.401
Species:Mus musculus
PMID:31122259
Title:miR-301a promotes lung tumorigenesis by suppressing Runx3.
References for Fibronectin Rabbit pAb
Product:Fibronectin Rabbit pAb
Journal:Medical oncology (Northwood, London, England)
Application:WB, ICC
IF:3.064
Species:Homo sapiens
PMID:31407170
Title:EMT-related protein expression in polyploid giant cancer cells and their daughter cells with different passages after triptolide treatment.