Flag Tag

Cat Number: MAB-94573
Conjugate: Unconjugated
Size: 100 ug
Clone: 1E6
Host: Ms
Isotype: IgG1
Immunogen: Synthetic Peptide
Reactivity: All Species

Western Blot: 1:5000
Immunoprecipitation: 1:200
Immunofluorescence: 1:2000

Purification: The antibody was affinity-purified from mouse ascites by affinity chromatography using specific immunogen.

The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.

Western Blot (WB) analysis
of Recombinant protein,
diluted at 1) 1:2,500,
2) 1:5000


1ug Flag fusion protein+ Primary antibody dilution at 1:5000


IP antibody use: 5ug Flag Mouse IgG1 per ml Lysate, WB 1:5000
1) Untransfected 293 cell lysate
2) transfected 293 cell lysate with Flag-tag fusion protein
3) IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4) IP (transfected 293+anti- Flag mA)

Immunofluorescence (IF) analysis of 293 cells transfected with a Flag-tag protein,1:2000 dilution (blue DAPI, red anti-Flag)