FoxO1 (phospho Ser256) rabbit Polyclonal Antibody
| Cat Number: | ABP10446 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 ug |
| Clone: | Polyclonal |
| Concentration: | 1 mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272 |
| Reactivity: | Human;Mouse;Rat;Drosophila |
| Applications: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Molecular: | 82kD |
| Purification: | The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma |
| Background: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008], |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thawcycles |
Immunofluorescence analysis of Hela cell. 1,FoxO1 (phospho Ser256) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Active Caspase-3 Monoclonal Antibody(5E1)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalo
Western Blot analysis of HELA cells using Phospho-FoxO1 (S256) Polyclonal Antibody diluted at 1:1000
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FKHR (Phospho-Ser256) Antibody