FOXP3 Rabbit Monoclonal Antibody
| Cat Number: | MAB-94759 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC1159 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 286-431 of human FOXP3 |
| Reactivity: | Human |
| Applications: | Western Blot: 1:500 – 1:2000 Immunofluorescence: 1:200-1:500 |
| Molecular Weight: | 45KDa |
| Purification: | Affinity purification |
| Synonyms: | AIID; DIETER; IPEX; JM2; PIDX; XPID |
| Background: | The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of Jurkat cells, using FOXP3 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug FOXP3 antibody.
Western blot was performed from the immunoprecipitate using FOXP3 antibody at a dilution of 1:1000.
Immunofluorescence: FoxP3 Antibody Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.
Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).