FOXp3 Rabbit Monoclonal Antibody
| Cat Number: | MAB-94761 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC505 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 206-300 of human FOXP3 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:100 – 1:500 Immunohistochemistry: 1:50-1:200 |
| Molecular Weight: | 47KDa |
| Purification: | Affinity purification |
| Synonyms: | FOXP3;AIID;DIETER;IPEX;JM2;PIDX;XPID |
| Background: | The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Form: | Liquid |
| Buffer: | PBS with 0.01% thiomersal,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of Mouse thymus cells, using FOXP3 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Lymphocyto tissue, showing cytoplasmic and nuclear localization with DAB staining using FoxP3 Monoclonal Antibody.