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Frataxin

Cat Number: AB-83941
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 -1:1000
Immunohistochemistry: 1:50 – 1:100
Immunofluorescence: 1:50 -1:100

Molecular: 14kDa
Purification: Aff. Pur.
Synonyms: FXN; CyaY; FA; FARR; FRDA; X25; frataxin
Background:

This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
AB-83941-1.jpg

Western blot analysis of extracts of various cell lines, using FXN / Frataxin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

AB-83941-2.jpg

Immunohistochemistry of paraffin-embedded rat heart using FXN / Frataxin antibody at dilution of 1:100 (40x lens).

AB-83941-3.jpg

Immunofluorescence analysis of A549 cells using FXN / Frataxin antibody . Blue: DAPI for nuclear staining

AB-83941-4.jpg

Immunohistochemistry of paraffin-embedded mouse heart using FXN / Frataxin antibody at dilution of 1:100 (40x lens).