GAPDH
| Cat Number: | AB-81594 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2). |
| Reactivity: | Hu, Ho, Cw, Pg, Ch, Rt, Ms |
| Applications: | Western Blot: 1:1000 – 1:10000 |
| Molecular Weight: | 36kDa |
| Purification: | Aff. Pur. |
| Synonyms: | G3PD;GAPD;HEL-S-162eP;GAPDH |
| Background: | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigneda variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using GAPDH antibody at 1:10000
dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.
Immunohistochemistry of paraffin embedded rat kidney using GAPDH Rabbit pAb at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using GAPDH Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
References
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Oncology Letters
Application:WB
IF:1.39
Species:Homo sapiens
PMID:29552157
Title:Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Molecular Cell
Application:IF
IF:14.714
Species:Homo sapiens
PMID:29727616
Title:The BUB3-BUB1 Complex Promotes Telomere DNA Replication
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:biochemical and biophysical research communications
Application:WB
IF:2.559
Species:Homo sapiens
PMID:29605298
Title:Micro124-mediated AHR expression regulates the inflammatory response of chronic rhinosinusitis (CRS) with nasal polyps
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Inflammation
Application:WB, IF
IF:2.88
Species:Homo sapiens
PMID:30187339
Title:CDK5 Regulates PD-L1 Expression and Cell Maturation in Dendritic Cells of CRSwNP
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Oncology reports
Application:WB
IF:2.97
Species:Homo sapiens
PMID:30320343
Title:Phosphorylation of nucleolin is indispensable to its involvement in the proliferation and migration of non-small cell lung cancer cells.
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Inflammation
Application:WB
IF:2.88
Species:Homo sapiens
PMID:31041569
Title:Regulation of KDM2B and Brg1 on Inflammatory Response of Nasal Mucosa in CRSwNP
.
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Oncology reports
Application:WB
IF:3.04
Species:Homo sapiens
PMID:31115582
Title:Cytokeratin 18 regulates the transcription and alternative splicing of apoptotic related genes and pathways in HeLa cells.
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Nucleic Acids Research
Application:WB, IF
IF:11.14
Species:Homo sapiens
PMID:31970402
Title:Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:The EMBO Journal
Application:WB
IF:11.22
Species:Homo sapiens
PMID:32368828
Title:RNA m6 A methylation regulates sorafenib resistance in liver cancer through FOXO3-mediated autophagy
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Autophagy
Application:WB
IF:11.05
Species:Danio rerio
PMID:30205735
Title:CERKL regulates autophagy via the NAD-dependent deacetylase SIRT1
References for GAPDH Rabbit pAb
Product:GAPDH Rabbit pAb
Journal:Cells
Application:WB
IF:5.65
Species:Mus musculus
PMID:31614673
Title:Perilipin 5 Protects against Cellular Oxidative Stress by Enhancing Mitochondrial Function in HepG2 Cells