Top

GDF-5 Rabbit Polyclonal Antibody

Cat Number: AB-E7989
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human GDF5. AA range:361-410.
Reactivity: Human;Mouse;Rat
Applications:

Western Blot: 1/500 – 1/2000

Immunohistochemistry (paraffin-embedded tissues):  1/100-1/300

Molecular Weight: 55kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: GDF5; CDMP1; Growth/differentiation factor 5; GDF-5; Cartilage-derived morphogenetic protein 1; CDMP-1; Radotermin
Background:

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mutations in this gene are associated with acromesomelic dysplasia, brachydactyly, chondrodysplasia, multiple synostoses syndrome, proximal symphalangism, and susceptibility to osteoarthritis.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-E7989-1

Western Blot analysis of 293 cells using GDF-5 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

AB-E7989-2

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

AB-E7989-3

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

AB-E7989-4

Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100