GFAP Mouse Monoclonal Antibody
| Cat Number: | MABS15096 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Clone: | 5C10 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG1, κ |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse GFAP |
| Reactivity: | Species, Human, Mouse, Rat, Human, Mouse, Rat, Monkey, zebrafish |
| Applications: | WB 1: 500-1: 1000 IHC: 1: 500-1: 1000 IF 1: 500-1: 1000 |
| Molecular: | 49kDa |
| Purification: | Affinity purification |
| Synonyms: | GFAP, ALXDRD, glial fibrillary acidic protein, Astrocyte, Intermediate filament protein |
| Background: | Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells.GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin,desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of GFAP.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.
WB analysis of GFAP.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.
IHC analysis of GFAP.Sample: Human glioblastoma multiforme, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , pressure cooker 2 min.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 200, RT, 1h.