GFAP Rabbit Monoclonal Antibody
| Cat Number: | MABN21476 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 200μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG,Kappa |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:1000-1:5000,IHC 1:200-1:1000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200 |
| Molecular: | Calculated MW:50kD;Observed MW:50kD |
| Purification: | Affinity purification |
| Synonyms: | GFAP;Glial fibrillary acidic protein;GFAP |
| Background: | Cell localization:Cytoplasm.This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Form: | liquid |
| Buffer: | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Western blot analysis of lysates from Mouse brain cells, using GFAP Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue. 1, GFAP Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue. 1, GFAP Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).