Glutamate Receptor 2
| Cat Number: | AB-10699 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 99kDa |
| Purification: | Aff. Pur. |
| Background: | Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of GLU are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainite (glutamate receptor, ionotropic, AMPA 2; Jurkat; cell lysate ). Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human Glutamate Receptor 2 |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using GluR2 Rabbit) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.
Immunofluorescence using
Glutamate Receptor 2.
Immunohistochemistry of paraffin-embedded
rat brain using GluR2 Rabbit antibody
at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded
mouse brain using GluR2 Rabbit Antibody at dilution
of 1:100 (40xlens).