Glutamate Transporter 2 (GLT1/EAAT2) Rabbit Polyclonal Antibody
| Cat Number: | AB-82459 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human EAAT2 /GLT-1/ SLC1A2. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:2,500 |
| Molecular Weight: | 65 kDA |
| Purification: | Protein A affinity purified |
| Background: | This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. |
| Form: | Liquid |
| Buffer: | PBS (pH7.4) with 0.02% sodium azide, 50% glycerol pH 7.3. |
| Storage: | Ship at 2-8°C . Store at RT for short term. Store at -20°C for one year. Avoid repeated freeze and thaw cycles. |
Western blot analysis of extracts of various cell lines, using GLT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using GLT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using GLT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using GLT1 antibody at dilution of 1:100 (40x lens).