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GRP-94

Cat Number: AB-83479
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1.
Reactivity: Hu,Ms,Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:100
Immunofluorescence: 1:50 – 1:100

Molecular: 110kDa
Purification: Aff. Pur.
Synonyms: HSP90B1;ECGP;GP96;GRP94;HEL-S-125m;HEL35;TRA1
Background:

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5′ exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
AB-83479-1.jpg

Western blot analysis of extracts of various cell lines, using GRP-94 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.

AB-83479-2.jpg

Immunohistochemistry of paraffinembedded
rat testis using GRP-94
antibody at dilution of 1:200 (40x lens).

AB-83479-3.jpg

Immunohistochemistry of paraffinembedded
human lung cancer using
GRP-94 antibody at dilution of 1:200 (40x lens).

AB-83479-4.jpg

Immunofluorescence analysis of HeLa cells using GRP-94 antibody at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.

AB-83479-5.jpg

Immunofluorescence analysis of NIH/3T3 cells using GRP-94 antibody at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.

AB-83479-6.jpg

Immunofluorescence analysis of PC-12
cells using GRP-94 antibody at
dilution of 1:100 (40x lens).
Blue: DAPI for
nuclear staining.

References

References for HSP90B1 Rabbit pAb
Journal:VIRUSES-BASEL
Application:WB
IF:.464
Species:Sus scrofa
PMID:27916855
Title:Comparative Proteome Analysis of Porcine Jejunum Tissues in Response to a Virulent Strain of Porcine Epidemic Diarrhea Virus and Its Attenuated Strain

References for HSP90B1 Rabbit pAb
Journal:Experimental cell research
Application:WB
IF:3.32
Species:Mus musculus
PMID:31202710
Title:The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.