|Immunogen:||A synthetic peptide corresponding to HA tag.|
Western Blot: 1:2000 – 1:5000
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
|Buffer:||PBS with 0.02% sodium azide,50% glycerol,pH7.3.|
|Storage:||Store at -20°C for 1 year. Avoid repeated freeze and thaw cycles.|
Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) t 1:10000 dilution.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.
Immunofluorescence analysis of 293T cells
transfected with HA-Tag fusion protein and
untreated 293T cells use Mouse anti HATag
mAb at dilution of 1:50 (40xlens).
Blue: DAPI for nuclear staining.
Immunoprecipitation of over-expressed
HA-tagged protein in 293T cells using HAtag
antibody . A mock served as
negative control and over-expressed 293T
cell lysate served as positive control.