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HE4/WFDC2 Rabbit Monoclonal Antibody

Cat Number: MAB-95046
Conjugate: Unconjugated
Size: 100 ug
Clone: ARC1936
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 45-124 of human HE4/WFDC2.
Reactivity: Human, Mouse
Applications:

Western Blot:    1:500 – 1:2000
Immunofluorescence:    1:50 – 1:200

Immunocytcohemistry:  1:50 – 1:200

Molecular Weight: 22 kDa
Purification: Affinity purification
Synonyms: HE4; WAP5; EDDM4; dJ461P17.6; HE4/WFDC2
Background:

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-95046-1

Western blot analysis of extracts of various cell lines, using HE4/WFDC2 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.

MAB-95046-2

Immunofluorescence analysis of NIH-3T3 cells using HE4/WFDC2 Rabbit mAb  at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

MAB-95046-3

Immunofluorescence analysis of U-2 OS cells using HE4/WFDC2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.