HE4/WFDC2 Rabbit Monoclonal Antibody
Cat Number: | MAB-95046 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | ARC1936 |
Concentration: | 1mg/ml |
Host: | Rabbit |
Isotype: | IgG |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 45-124 of human HE4/WFDC2. |
Reactivity: | Human, Mouse |
Applications: | Western Blot: 1:500 – 1:2000 Immunocytcohemistry: 1:50 – 1:200 |
Molecular Weight: | 22 kDa |
Purification: | Affinity purification |
Synonyms: | HE4; WAP5; EDDM4; dJ461P17.6; HE4/WFDC2 |
Background: | This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. |
Form: | Liquid |
Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |

Western blot analysis of extracts of various cell lines, using HE4/WFDC2 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.

Immunofluorescence analysis of NIH-3T3 cells using HE4/WFDC2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using HE4/WFDC2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.