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Heparanase 1

Cat Number: AB-84148
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human Heparanase 1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry: 1:50 – 1:100
Immunofluorescence: 1:50 – 1:100

Molecular: 50kDa/65kDa
Purification: Aff. Pur.
Synonyms: HPSE; HPA; HPA1; HPR1; HPSE1; HSE1; heparinase
Background:

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84148-1.jpg

Western blot analysis of extracts of
various cells, using Heparanase 1
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

AB-84148-2.jpg

Immunofluorescence analysis of HeLa
cells using Heparanase 1 antibody
at dilution of 1:100. Blue:
DAPI for nuclear staining.

AB-84148-3.jpg

Immunofluorescence analysis of
NIH/3T3 cells using Heparanase 1
antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.