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HER4 / ERBB4

Cat Number: AB-81617
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-160 of human ErbB4/HER4/HER4.
Reactivity: Hu, Ms, Rt
Applications:

WB: 1:500 – 1:1000
IHC: 1:50 – 1:100
IF: 1:50 – 1:100

Molecular: 180kDa
Purification: Aff. Pur.
Synonyms: ALS19;HER4;p180erbB4;ERBB4;HER4/ErbB4
Background:

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
AB-81617-1.jpg

Western blot analysis of extracts of mouse brain, using ErbB4/HER4/HER4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-81617-2.jpg

Immunohistochemistry of paraffin embedded human placenta using
ErbB4/HER4/HER4 Rabbit pAb at
dilution of 1:100 (40x lens).

AB-81617-3.jpg

Immunofluorescence analysis of C6 cells using ErbB4/HER4/HER4 Rabbit pAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.