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HIF-1alpha

Cat Number: AB-80484
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide of human HIF1A
Reactivity: Hu, Ms
Applications:

Western Blot: 1:500- 1:1,000
Immunofluorescence (on cells): 1:50- 1:100

Molecular: 105kDa
Purification: Aff. Pur.
Synonyms: HIF1A; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; hypoxia-inducible factor 1-alpha
Background:

HIF-1 alpha(Hypoxia-inducible factor 1 alpha,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1 alpha transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1 alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1 alpha regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
AB-80484-1.jpg

Western blot analysis of extracts of HepG2 cells, using HIF1A antibody at 1:1,000 dilution.HepG2 cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 15s.

AB-80484-2.jpg

Immunofluorescence analysis of U2OS cells using HIF1A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.