His Tag (HRP Conj.)
| Cat Number: | MAB-91880HRP |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | B8 |
| Concentration: | 1mg/mL |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | A synthetic peptide of His-Tag |
| Reactivity: | All Species |
| Applications: | • Western Blot (1:5000) |
| Purification: | Affinity purification |
| Background: | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-His antibodies. |
| Form: | Liquid |
| Buffer: | PBS, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C for 1 year. Store at -80°C for long term. Avoid repeated freeze and thaw cycles. |
Western blot analysis of recombinant His Tag protein using HRP-conj. Mouse anti His-Tag at dilution of 1:5000.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.