His Tag (HRP Conj.)

Cat Number: MAB-91880HRP
Conjugate: Unconjugated
Size: 100 ug
Clone: B8
Concentration: 1mg/mL
Host: Ms
Isotype: IgG1
Immunogen: A synthetic peptide of His-Tag
Reactivity: All Species

• Western Blot (1:5000)

Purification: Affinity purification

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-His antibodies.

Form: Liquid
Buffer: PBS, 50% glycerol, pH7.3.
Storage: Store at -20°C for 1 year. Store at -80°C for long term. Avoid repeated freeze and thaw cycles.

Western blot analysis of recombinant His Tag protein using HRP-conj. Mouse anti His-Tag at dilution of 1:5000.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.