Histone H3 Rabbit Monoclonal Antibody
| Cat Number: | MABN21336 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μL |
| Clone: | D1H2 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG,Kappa |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:5000-1:20000,IHC 1:5000-1:20000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200 |
| Molecular: | Calculated MW:15kD;Observed MW:15kD |
| Purification: | Affinity purification |
| Synonyms: | HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3 |
| Background: | Cell localization:Nucleus.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015], |
| Form: | liquid |
| Buffer: | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Western blot analysis of lysates from Hela cells, using Histone H3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.