Histone H3 Rabbit Polyclonal Antibody
| Cat Number: | ABE1054 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3. |
| Reactivity: | Human,Mouse,Rat,Other (Wide Range Predicted) |
| Applications: | Western Blot: 1:2000 – 1:10000 Immunocytochemistry: 1:50 – 1:200 Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 17kDa |
| Purification: | Affinity purification |
| Synonyms: | H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Histone H3 |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various lysates using Histone H3 Rabbit pAb) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using Histone H3 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed
with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver using Histone H3 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human brain using Histone H3 Rabbit pAbat dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Histone H3 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse testis using Histone H3 Rabbit pAb at dilution of 1:200 (40xlens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IFstaining protocol.Immunofluorescence analysis of paraffin-embedded rat testis using Histone H3 Rabbit pAb at dilution of 1:200 (40xlens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody . Western blot was performed from the immunoprecipitate using
Histone H3 antibody at a dilution of 1:30000.
Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody . Western blot was performed from the immunoprecipitate using
Histone H3 antibody at a dilution of 1:30000.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using Histone H3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked
by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.