HSL
| Cat Number: | AB-84408 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human LIPE. |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 81kDa |
| Purification: | Aff. Pur. |
| Synonyms: | LIPE;AOMS4;FPLD6;HSL;LHS |
| Background: | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines,
using HSL antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG
(H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
References
References for HSL Rabbit pAb
Product:HSL Rabbit pAb
Journal:Journal of agricultural and food chemistry
Application:WB
IF:5.279
Species:Mus musculus
PMID:31075194
Title:A Combination of Capsaicin and Capsiate Induces Browning in 3T3-L1 White Adipocytes via Activation of the PPARγ/ β3-AR Signaling Pathways.