HSP90
| Cat Number: | AB-80156 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide of Human HSP90 |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot : 1:1000 |
| Molecular Weight: | 101kDa |
| Purification: | Aff. Pur. |
| Synonyms: | HSP90AA1; EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2; heat shock protein HSP 90-alpha |
| Background: | The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell line, using HSP90
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Kit
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human prostate using HSP90 – dilution: 1:100
Immunohistochemistry of paraffin-embedded human prostate using HSP90 – dilution: 1:100
Immunohistochemistry of paraffin-embedded human lung using HSP90 – dilution: 1:100
Immunofluorescence using HSP90 on NIH-3T3 cells– dilution: 1:100
Blue: DAPI for nuclear staining.