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HSP90 alpha Rabbit Monoclonal Antibody

Cat Number: MAB-80112
Conjugate: Unconjugated
Size: 100 ug
Clone: SJ-90
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hsp90α
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500 – 1:2000
Immunoprecipitation: 1:500 – 1:1000
Molecular Weight: 90KDa
Purification: Purified
Synonyms: EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2
Background:

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.
Form: Liquid
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-80112-1

Western blot analysis of extracts of various cell lines, using Hsp90α Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.

MAB-80112-2

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Hsp90α antibody.
Western blot was performed from the immunoprecipitate using Hsp90α antibody at a dilution of 1:1000.

References:

Product:Hsp90α Rabbit mAb
Journal:Cell reports
Application:Western Blot
IF:0
Species:Mus musculus
PMID:36198274
Title:Destabilization of TP53 by USP10 is essential for neonatal autophagy and survival

References:

References for Hsp90α Rabbit mAb
Product:Hsp90α Rabbit mAb
Journal:PeerJ
Application:WB
IF:3.06
Species:Homo sapiens
PMID:36632140
Title:A novel fatty acid metabolism-related gene prognostic signature and candidate drugs for patients with hepatocellular carcinoma – PMC