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HSP90

Cat Number: AB-80156
Conjugate: Unconjugated
Size: 100 ul
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide of Human HSP90
Reactivity: Hu, Ms
Applications:

Western Blot : 1:1000
Immunohistochemistry: 1:50 – 1:100
Immunofluorescence: 1:50 – 1:100

Molecular: 101kDa
Purification: Aff. Pur.
Synonyms: HSP90AA1; EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2; heat shock protein HSP 90-alpha
Background:

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of various cell line, using HSP90

Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Kit
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human prostate using HSP90 – dilution: 1:100

Immunohistochemistry of paraffin-embedded human prostate using HSP90 – dilution: 1:100

Immunohistochemistry of paraffin-embedded human lung using HSP90 – dilution: 1:100

Immunofluorescence using HSP90 on NIH-3T3 cells– dilution: 1:100
Blue: DAPI for nuclear staining.